World No. 138 Alexandra Sasnovich of Belarus suffered a 4-6, 6-7 (2-7) defeat from Madison Brengle of the United States (151) at the Wimbledon qualifying round two on 18 June.
In her opener on the grass courts of the All England Lawn Tennis and Croquet Club the Belarusian took a confident win against Russia's Ksenia Pervak (140).
In the men's singles Vladimir Ignatik of Belarus (210) also completed his performance falling 6-1, 3-6, 4-6 to Spain's Adrian Menendez-Maceiras (172) in the second qualifying round. In the opener the Belarusian beat Joao Souza of Brazil (134) in two straight sets 6-2, 6-2.
Three qualifying rounds are needed to reach main-draw places.
The Wimbledon main-draw matches will kick off on 23 June, finish on 6 July.